Composite

Part:BBa_K3777025:Design

Designed by: SiYuan Peng   Group: iGEM21_OUC-China   (2021-10-01)


KB2-CtcS-T7(ctcO)-3WJdB-PctcS-sgRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 260
    Illegal NheI site found at 283
    Illegal NheI site found at 1164
    Illegal NotI site found at 832
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 121
    Illegal XhoI site found at 841
    Illegal XhoI site found at 1305
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 576


Design Notes

1


Source

1

References